Which observation indicates lipase activity in the tubes?

Which observation indicates lipase activity in the tubes?

• A. Increase in pH
• B. No change in pH
• C. Temperature drop
• D. Decrease in pH

Answer: (D)

Lipase breaks down fats into fatty acids and glycerol, and those fatty acids increase the acidity of the solution. In the tubes, seeing a drop in pH means fatty acids are being released, which shows the enzyme is active. If the pH doesn’t change, there’s no hydrolysis happening; a temperature drop isn’t a direct result of lipase action, and a rise in pH would imply basic conditions or loss of acid, not fatty acid production. So the observed decrease in pH best indicates lipase activity.

Lipase breaks down fats into fatty acids and glycerol, and those fatty acids increase the acidity of the solution. In the tubes, seeing a drop in pH means fatty acids are being released, which shows the enzyme is active. If the pH doesn’t change, there’s no hydrolysis happening; a temperature drop isn’t a direct result of lipase action, and a rise in pH would imply basic conditions or loss of acid, not fatty acid production. So the observed decrease in pH best indicates lipase activity.